phenol red free cell culture medium without glucose Search Results


low-density percoll solution  (Pharmacia Upjohn LLC)
90
Pharmacia Upjohn LLC low-density percoll solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Low Density Percoll Solution, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/low+density+percoll+solution/pmc02327129-112-9-12
Average 90 stars, based on 1 article reviews
low-density percoll solution - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
faces permabilization solution becton & dickinson  (Becton Dickinson)
90
Becton Dickinson faces permabilization solution becton & dickinson
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Faces Permabilization Solution Becton & Dickinson, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/faces+permabilization+solution+becton+++dickinson/us07897357-312-10-13
Average 90 stars, based on 1 article reviews
faces permabilization solution becton & dickinson - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
accumax cell dissociation solution  (PAN - Biotech)
90
PAN - Biotech accumax cell dissociation solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Accumax Cell Dissociation Solution, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/accumax+cell+dissociation+solution/pmc11275110-131-17-21
Average 90 stars, based on 1 article reviews
accumax cell dissociation solution - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
matrispace cell release solution  (Becton Dickinson)
90
Becton Dickinson matrispace cell release solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Matrispace Cell Release Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/matrispace+cell+release+solution/10__1161_slash_01__cir__0000163566__07427__73-51-22-26
Average 90 stars, based on 1 article reviews
matrispace cell release solution - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
cytofix/cytoperm™ 399 fixation/permeabilization solution kit  (Becton Dickinson)
90
Becton Dickinson cytofix/cytoperm™ 399 fixation/permeabilization solution kit
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Cytofix/Cytoperm™ 399 Fixation/Permeabilization Solution Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/cytofix+cytoperm++399+fixation+permeabilization+solution+kit/ppr0375612-242-5-10
Average 90 stars, based on 1 article reviews
cytofix/cytoperm™ 399 fixation/permeabilization solution kit - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
acutase cell suspension solution  (Corning Life Sciences)
90
Corning Life Sciences acutase cell suspension solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Acutase Cell Suspension Solution, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/acutase+cell+suspension+solution/bio_rxiv__2020__04__10__036483-113-14-15
Average 90 stars, based on 1 article reviews
acutase cell suspension solution - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
cell lysis solution wizard genomic dna purification kit  (Promega)
90
Promega cell lysis solution wizard genomic dna purification kit
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Cell Lysis Solution Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/cell+lysis+solution+wizard+genomic+dna+purification+kit/pmc12041197-260-28-33
Average 90 stars, based on 1 article reviews
cell lysis solution wizard genomic dna purification kit - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
lymphocyte isolation solution lymphoprep  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc lymphocyte isolation solution lymphoprep
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Lymphocyte Isolation Solution Lymphoprep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/lymphocyte+isolation+solution+lymphoprep/pm38664838-48-46-50
Average 90 stars, based on 1 article reviews
lymphocyte isolation solution lymphoprep - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
rbc lysis buffer solution  (Thermo Fisher)
99
Thermo Fisher rbc lysis buffer solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Rbc Lysis Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/RBC+lysis+buffer+for+human/pm38001638-63-16-21
Average 99 stars, based on 1 article reviews
rbc lysis buffer solution - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier
cell lysis solution  (Promega)
90
Promega cell lysis solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Cell Lysis Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/cell+lysis+solution/pm22548327-112-16-16
Average 90 stars, based on 1 article reviews
cell lysis solution - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
cck 8 solution  (Dojindo Labs)
99
Dojindo Labs cck 8 solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Cck 8 Solution, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/Cell+Counting+Kit-8/pmc12750810-72-26-28
Average 99 stars, based on 1 article reviews
cck 8 solution - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier
directpcr lysis reagent (cell) + proteinase k solution  (ViaGen Inc)
90
ViaGen Inc directpcr lysis reagent (cell) + proteinase k solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Directpcr Lysis Reagent (Cell) + Proteinase K Solution, supplied by ViaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phenol+red+free+cell+culture+medium+without+glucose/directpcr+lysis+reagent/pmc09543708-87-13-21
Average 90 stars, based on 1 article reviews
directpcr lysis reagent (cell) + proteinase k solution - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using Percoll solution 1·075 g/dl, layered over 1·035 g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).

Journal:

Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator

doi: 10.1046/j.1365-2567.2000.00934.x

Figure Lengend Snippet: CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using Percoll solution 1·075 g/dl, layered over 1·035 g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).

Article Snippet: The cells were then separated on density fractions using low‐density Percoll solution (Pharmacia & Upjohn, Peapack, WN) (1·035 g/dl), layered over a high‐density solution (1·075 g/dl), and centrifuged at 570 g for 20 min. Interface cells were collected and placed in 10‐ml Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded.

Techniques: Injection, Incubation, Positive Control, In Vitro